MicroSet 40x1024x - Microscope BRESSER - Free user manual and instructions

USER MANUAL MicroSet 40x1024x BRESSER

Operating Instructions

DISPOSAL Dispose of the packaging material/s as legally required. Consult the local au- thority on the matter if necessary. Do not dispose of electrical equipment in your ordinary refuse. The European guideline 2002/96/EU on Electronic and Electrical Equipment Waste and relevant laws applying to it require such used equip- ment to be separately collected and recycled in an environment-friendly manner. Empty bat- teries and accumulators must be disposed of separately. Information on disposing of all such equipment made after 01 June 2006 can be obtained from your local authority. EC Declaration of Conformity Bresser GmbH has issued a ‘Decla- ration of Conformity’ in accordance with applicable guidelines and corresponding standards. This can be viewed any time upon request.12 Here are the parts of your microscope (Fig. 1-6): 1 10x WF Eyepiece 2 16x WF Eyepiece 3 Barlow Lens 4 Compartment for optional accessories 5 Eyepiece supports 6 Microscope Head 7 Set Screw 8 Objective Nosepiece 9 Objective 10 Clips 11 Microscope Stage 12 LED Illumination (transmitted light) 13 Microscope Base 14 Selection Knob for Illumination 15 Battery compartment (3x AA) 16 Focus knob 17 Color Filter 18 LED Illumination (refl ected light) 19 Compartment for optional accessories 20 5 Slides, 10 Cover Sips and 5 Prepared Specimens plastic box 21 Specimens: a) Yeast b) “Gum Media” Glue c) Sea Salt d) Shrimp Eggs 22 Smartphone holder 23 Specimen Slicer 24 Shrimp Hatchery 25 Tweezers 26 Pipette 27 Carrying Case How do I use my microscope? Before you assemble your microscope, make sure that the table, desk or whatever surface that you want to place it on is stable, and does not wobble. How do I operate the electric LED illumination? The batteries supplied should be inserted into the battery compartment (15) located in the base plate before use. Remove the battery compartment cover by pressing lightly on the cover clip. Insert the batteries (3x AA) into the holder. There are two lights on the microscope. They do not use light bulbs, but rather light-emitting diodes (LED). The fi rst lamp shines onto the specimen from below and the second from above. (The thing that you want to observe with the microscope is called the object or specimen, by the way.) You can use each lamp on its own, or both of them together. There is a selection knob for this (Fig. 1, 14). It has three numbers: I, II and III. If you select the … I, the light only comes from below (transmit- ted light). II, the light only comes from above (refl ected light). III, both lamps shine light on the specimen. For transparent objects (transmitted-light ob- jects), number I is best. In order to observe fi rm, non-transparent objects (direct-light ob- jects), select number II. For semi-transparent objects, it is best to select number III. It is not recommended to use number III for transmitted-light objects on slides, since the light may cause refl ections on the surface of the slide, which will disturb your observation. When do I use the color fi lters? The color fi lters (Fig. 1, 17) are located below the microscope stage (Fig. 1, 11). They help you when you are observing very bright or clear specimens. Here, you can choose from various colors. This helps you better recog- nize the components of colorless or transpar- ent objects (e.g. grains of starch, protozoa).13

How do I adjust my microscope correctly? First, loosen the screw (Fig. 1, 7) and turn the microscope head (Fig. 1, 6) into a comfort- able viewing position. Each observation starts with the lowest mag- nifi cation. Adjust the microscope stage (Fig. 1, 11) so that it goes all the way down to the lowest position. Then, turn the objective nosepiece (Fig. 1, 8) until it clicks into place at the lowest magnifi cation (objective 4x). Note: Before you change the objective setting, al- ways move the microscope stage (Fig. 1, 11) to its lowest position. This way, you can avoid causing any damage! Now, insert the 10x eyepiece (Fig. 1, 1) into the Barlow lens (Fig. 1, 3). Make sure that the Barlow lens is placed all the way into the eyepiece sup- ports (Fig. 1, 5) and is not pulled out (Fig. 2). How do I observe the specimen? After you have assembled the microscope with the adequate illumination and adjusted it correctly, the following basic rules are to be observed: Start with a simple observation at the lowest magnifi cation. This way, it is easier to position the object in the middle (centering) and make the image sharp (focusing). The higher the magnifi cation, the more light you will require for a good image quality. Now place the pre- pared specimen (Fig. 6, 20) directly under the objective on the microscope stage (Fig. 3). The object should be located di- rectly over the illumination (Fig. 1, 12). In the next step, take a look through the eye- piece (Fig. 1, 1) and carefully turn the focus knob (Fig. 1, 16) until the image appears clear and sharp. Now you can select a higher magnifi cation by slowly removing the Barlow lens (Fig. 1, 3) from the eyepiece support (Fig. 1, 5). When the Barlow lens is almost completely pulled out, the magnifi cation can be increased to al- most double. If you would like an even higher level of mag- nifi cation, insert the 16x eyepiece (Fig. 1, 2) and turn the objective nosepiece (Fig. 1, 8) to a higher setting (10x or 40x). Important tip: The highest magnifi cation is not always the best for every specimen! Note: Each time the magnifi cation changes (eye- piece or objective change, pulling out the Barlow lens), the image sharpness must be readjusted with the focus knob (Fig. 1, 16). When doing this, make sure to be careful. If you move the microscope stage too quickly, the objective and the slide could come into contact and become damaged! Which light for which specimen? With this unit, a refl ected light and transmitted light microscope, you can observe transpar- ent, semi-transparent as well as non-transpar- ent objects. The image of the given object of observation is “transported” through the light. As a result, only the correct light will allow you to see something! If you are observing non-transparent (opaque) objects (e.g. small animals, plant compo- nents, stones, coins, etc.) with this micro- scope, the light falls on the object that is be- ing observed. Fig. 3

B/C14 From there, the light is refl ected back and passes through the objective and eyepiece (where it gets magnifi ed) into the eye. This is refl ected light microscopy. For transparent objections (e.g. protozoa), on the other hand, the light shines from below, through the opening in the microscope stage and then through the object. The light travels further through the objective and eyepiece, where it is also magnifi ed, and fi nally goes into the eye. This is transmitted- light microscopy. Many microorganisms in water, many plan components and the smallest animal parts are already transparent in nature. Others have to be prepared. We may make them transpar- ent through a treatment or penetration with the right materials (media), or by taking the thinnest slices from them (using our hand or a specimen slicer), and then examine them. You can read more about this in the following sections. How do I make thin specimen slices? Only do this with the supervision of your par- ents or another adult. As I already pointed out, the thinnest slices possible are taken from an object. In order to get the best results, we need some wax or paraffi n. It is best if you get a candle. Place the wax in a pot and heat it carefully over a low burner. Now, dip the object in the liquid wax a few times. Then, let the wax get hard. Using the specimen slicer (Fig. 6, 23) or a knife/scalpel, cut the smallest slices from the object that is covered with wax. These slices are to be laid on a slide and covered with a cover slip. How do I make my own specimens? Fig. 7 Fig. 8

Take the object that you want to observe and place it on a glass slide (Fig. 6, 20). Then, add a few drops of distilled water on the ob- ject (Fig. 7) using a pipette (Fig. 7, 26). Now, place a cover slip vertically at the edge of the drop of water, so that the water runs along the edge of the cover slip. Then, slowly lower the cover slip over the water drops (Fig. 8). Note: The included glue “gum media” (Fig. 5, 21b) is used to make permanent prepared speci- mens. Use this in place of the distilled water. If you want to keep the object in place on the slide permanently, use the gum media. Smartphone holder Attach the smartphone holder (22) to the eyepiece. The suction cups must be clean and free from dust and dirt. A slight moiste- ning is helpful. Now press your smartphone on the retai- ning plate and make sure that it is properly secured. As a backup, you should secure it with the enclosed rubber strap. Smartphones with a rough surface will not hold as good as smartphones with a smooth surface. Now start the Camera app. The camera needs to rest just above the eyepiece. Center the smartphone exactly over the eyepiece, so the image can be seen precise- ly centered on your screen. In some cases you need to adjust with the zoom function to display the image fullscreen. A light shading at the edges is possible. Take the smartphone carefully off the holder after use. NOTE: Make sure that the smartphone can not slip out of the holder. Bresser GmbH assumes no liability for any damages caused by a dropped smartphone.15

• Readjust focus Image fl ickers (while observing with optional available MicrOcular) = Monitor refresh rate not adequate