Bio Science Trino - Microscope BRESSER - Free user manual and instructions

USER MANUAL Bio Science Trino BRESSER

Do not dispose of electric equipment together with household waste material! In observance of European Directive 2002/96/EC on waste electrical and electronic equipment (WEEE) and its implementation in accordance with national law, electric equipment that have reached the end of its life must be collected separately and returned to an environmentally compatible recycling facility. Discharged batteries and damaged re-chargable batteries must be disposed of at special battery collection points. Information is available from your local disposal agent or local authority.

To work with this microscope, sharp and pointed aids are being used. Please take care that this microscope and its accessories are stored out of the reach of children. Let children only work with this microscope under an adult's supervision! Keep packing material (plastic bags etc.) away from children!

Table of Contents

I. Components

II. Technical Data / Included with Your Purchase
III. Observing Operation
IV. The Operations of Each Unit
V. Maintenance, EEC Conformity Explanation, Warranty

I. COMPONENTS

Fig. 1

Nosepiece

Condenser up-down knob

Knob for tensional adjustment of focusing

Coarse focusing knob

Fine focusing knob

Power switch, Brightness control knob

Power supply

Auxiliary lens of condenser

Condenser holding screw

Condenser centering screw

Stage

Objective

Fig. 2

Eyepiece

Specimen holder

Condenser (with aperture diaphragm)

Field diaphragm and filter seat

Limit knob (up stop)

Fine focusing knob

Power supply

AC-adapter

Transversal adjustment knob

Longitudinal adjustment knob

Tube holding screw

Microscope head (Binocular or Trinocular)

Fig. 3

Tube holding screw

Microscope head (Binocular or Trinocular)

Eyepiece

Nosepiece

Objective

Condenser centering screw

Condenser holding screw

Condenser (with aperture diaphragm)

II. TECHNICAL DATA / INCLUDED WITH YOUR PURCHASE
Eyepieces
Eyepiece type Magnification Field of view			Focal length	Remarks
Wide field eyepiece (WF) 10x 20 25		diameter / mm	/ mm
WF eyepiece with crosshair 10x optional

Objectives
Objective type Magnification Numerical aperture		NA	Working distanceWD / mm
Achromaticobjective	4x 0.1 17.4
	10x 0.25 6.6
	40x 0.65	0.64
	100x	1.25 (oil)	0.19

Total magnification
Objective:	4x	10x	40x	100x (oil)
Total magnification
Eyepiece:
10x 40x	100x	400x	1000x

traverse 75~mm

adjustment.

III. OBSERVING OPERATION

1. Turn down the power switch in order to switch on the illumination. The power switch also works as a brightness control knob.
2. By turning the nosepiece, set the 10x objective into the operation position. Then focus the specimen, which is on the stage.
3. Adjust the interpupillary distance and the diopter (for binocular observation).
4. Adjust the up & down position of the condenser, the light control and the aperture diaphragm in order to get a satisfying luminal effect. When you watch with the 4x or 10x objective, bring down the condenser to get symmetrical light. When you watch with the 4x objective, put the auxiliary lens of condenser in observation position.
5. When you interchange objectives, turn the nosepiece and refocus slightly with the fine focusing knob. When you use the 100x objective, be sure to put a drop of immersion oil between the objective and the specimen as well as between the condenser and the slide.

IV. THE OPERATIONS OF EACH UNIT

1. Adjustment of interpupillary distance (Fig. 4)

Fig. 4

Put the specimen on the stage and bring it into the exact focus. Adjust the interpupillary distance of the binocular viewing by "folding" the binocular tube (1) on the optical head, until the right and the left field of view can be composed to one.

2. Adjustment of diopter (Fig. 4)

Put the specimen on the stage. Turn the 40x objective to the working position. Firstly, observe at the right eyepiece with the right eye; adjust the coarse and fine focusing knob to image clearly. Secondly, observe at the left eyepiece with the left eye; adjust the diopter control (2) to image clearly.

3. Coarse/Fine focusing (Fig. 5)

Fig. 5

The instrument uses a coaxial coarse/fine focusing mechanism. The knob for tensional adjustment of focusing (4) is used for adjusting the tension of the coarse focusing knob (3) to prevent the stage from naturally sliding down. The limit knob (1) prevents accidental contact between specimen and objective. After locking the limit knob (by turning up and fastening) at a chosen height of the stage, you cannot focus the object closer to the objective using the coarse focusing knob. So, the object is protected against damage. (2) is the fine focusing knob.

4. Stage (Fig. 6)

Fig. 6

The convenient specimen holder (1) on the stage is used for holding the slide glass (2). The longitudinal (lengthwise) adjustment knob (3) and the transversal (cross) adjustment knob (4) are coaxial. The stage moves expediently.

5. Adjustable Abbe condenser (Fig. 7)

Fig. 7

The condenser is moved up or down via turning the up-down knob (1). For centering, the condenser centering screws (6) are used. The condenser can be taken down easily if one unscrews the condenser holding screw (2). The place for a filter plate is on the filter seat (5).

6. Power switch and adjustable brightness (Fig. 7)

Turn down the power switch (3). Adjust the light control (4) until the image can be observed comfortably. Note: Don't let the light control at the position of maximum brightness for a long time. That reduces the life-span of the lamp.

7. Kohler illumination (Fig. 7)

The Kohler illumination is the optimal microscopic illumination and therefore standard for scientific research and microphotography. One gets it using the fixed field diaphragm and the height- and center-adjustable Abbe condenser:

a) Using the condenser up-down knob (1), move the condenser (Fig. 3, No. 8) to the highest position, right under the stage.
b) Turn on the power switch (3) and focus the object.
c) Shut the field diaphragm (5) as close as possible. If the image of the field diaphragm lies out of the field of view, move it into the field using the condenser centering screws (6).
d) Using the condenser up-down knob (1), change the height of the condenser, until the image of the field diaphragm is clear.
e) Using the condenser centering screws (6), center the image of the field diaphragm in the field of view.
f) Open the field diaphragm so widely, that its edge has only just left the field of view and this field is complete illuminated. It may be, that you have to center the condenser a little bit again. Now, adjust the aperture diaphragm, which is described in the next paragraph.

8. Aperture diaphragm (Fig. 8)

Fig. 8

The aperture diaphragm lever (Fig. 7, No. 4) can be turned in order to open or close the aperture diaphragm. Remove the eyepieces and watch through the eyepiece tube. For centering, the condenser centering screws (Fig. 7, No. 6) are used when the diaphragm image is eccentric with the objective pupil (1). Turn the aperture diaphragm lever for getting a good resolution and contrast perception. Usually, the diameter of the aperture diaphragm image (2), which has to be adjusted, is 70-80 percent of the objective pupil.

9. Exchange of the lamp (Fig. 9)

a) Switch off the power switch and pull out the plugs of the AC-adapter from mains socket and from mains in at the microscope (Fig. 2, No. 7).
b) Incline the microscope, loose the fixing screw (3) of the lamp door on the middle part of the bottom and open the lamp door; so, you remove the lamp baseboard (1) from the bottom.
c) Pull out the old lamp (4) from the lamp base (5). Be careful, as the lamp may be hot!
d) Insert the new lamp (4) into the lamp base (5). Notice the properly touching; take care not to touch the lamp with bare fingers. E. g., use the protective envelope of the lamp or a tissue, in order to grasp the bulb.

e) Reinstall the lamp door (1) with lamp base board (5) on the bottom with the screw (3).
f) After mounting the lamp well, plug in the power cord, turn on the power switch, turn the objective lens into the light path, adjust the condenser upwards and downwards, and make light enter the view field. If the light spot is offset from the center of view, loose the screw (2) slightly and move the lamp base (5). Move the lamp spot into the center, then tighten up the screw (2) immediately.

Fig. 9

V. MAINTENANCE

1. Sweep the lens

Sweep the lens by lens tissue or soft fabric immersed with a mixed liquid of alcohol/ether. Clean the 100x oil objective from oil whenever you finish operating.

2. Clean the painted parts

The dust on the painted parts can be removed by gauze. For the grease spots, the gauze immersed slightly with aviation gasoline is recommended. Do not use organic solvents such as alcohol, ether or other thinner etc. for cleaning the painted parts or plastic components.

3. Avoid disassembling the microscope

Because of being a precise instrument, do not disassemble the microscope casually. That may cause serious damage to its performance.

4. Being not used

Cover the microscope with the dust cover (made of polymethylmethacrylate or polyethylene) and place it there, where it is dry and mouldless. We suggest the storage of all objectives and eyepieces in a closed container with drying agent.

EEC CONFORMITY EXPLANATION

Bresser GmbH, resident in 46414 Rhede/Westf., Gutenbergstr. 2, Germany, explains the agreement with in the following specified EEC guidelines for this product:

EN 61326: 1997

EN 61000-3-2

Product description: Biological microscope

Rhede, July 20, 2007

Bresser GmbH

Helmut Ebbert

Managing director

ATTENTION!

Rhede, 20. July 2007

Bresser GmbH

Helmut Ebbert

Errors and technical changes reserved