BIOSCIENCE 401000X - Microscope BRESSER - Free user manual and instructions

USER MANUAL BIOSCIENCE 401000X BRESSER

II. Technical Data / Included with Your Purchase

III. Observing Operation

IV. The Operations of Each Unit

V. Maintenance, EEC Conformity Explanation, Warranty

Knob for tensional adjustment of focusing

Power switch, Brightness control knob

Auxiliary lens of condenser

Condenser holding screw

Condenser (with aperture diaphragm)

Field diaphragm and filter seat

Condenser holding screw

Condenser (with aperture diaphragm) Eyepiece type Magnification Field of view diameter / mm Focal length ƒ / mm Remarks Wide field eyepiece (WF) 10x 20 25 WF eyepiece with crosshair 10x optional Objective type Magnification Numerical aperture

• Stage: Travel range is longitudinal 50 mm, traverse 75 mm.

• Fine focusing knob: Minimum division 0.002 mm.

• Interpupillary distance adjustment range: 53-75 mm.

• Light sources: 12 V, 30 W halogen lamp with brightness adjustment.

• Filters: Transparent and blue.

CAUTION!! To work with this microscope, sharp and pointed aids are being used. Please take care that this microscope and its accessories are stored out of the reach of children. Let children only work with this microscope under an adult’s supervision! Keep packing material (plastic bags etc.) away from children! Objective: 4x 10x 40x 100x (oil) Total magnification Eyepiece: 10x 40x 100x 400x 1000x Total magnification9

III. OBSERVING OPERATION

1. Turn down the power switch in order to switch on the illumi-

nation. The power switch also works as a brightness control knob.

2. By turning the nosepiece, set the 10x objective into the ope-

ration position. Then focus the specimen, which is on the stage.

3. Adjust the interpupillary distance and the diopter (for binocular

4. Adjust the up & down position of the condenser, the light

control and the aperture diaphragm in order to get a satisfying luminal effect. When you watch with the 4x or 10x objective, bring down the condenser to get symmetrical light. When you watch with the 4x objective, put the auxiliary lens of conden- ser in observation position.

5. When you interchange objectives, turn the nosepiece and

refocus slightly with the fine focusing knob. When you use the 100x objective, be sure to put a drop of immersion oil between the objective and the specimen as well as between the con- denser and the slide.

IV. THE OPERATIONS OF EACH UNIT

1. Adjustment of interpupillary distance (Fig. 4)

Fig. 4 Put the specimen on the stage and bring it into the exact focus. Adjust the interpupillary distance of the binocular viewing by “folding” the binocular tube (1) on the optical head, until the right and the left field of view can be composed to one.

2. Adjustment of diopter (Fig. 4)

Put the specimen on the stage. Turn the 40x objective to the working position. Firstly, observe at the right eyepiece with the right eye; adjust the coarse and fine focusing knob to image clearly. Secondly, observe at the left eyepiece with the left eye; adjust the diopter control (2) to image clearly.

3. Coarse/Fine focusing (Fig. 5)

Fig. 5 The instrument uses a coaxial coarse/fine focusing mecha- nism. The knob for tensional adjustment of focusing (4) is used for adjusting the tension of the coarse focusing knob (3) to prevent the stage from naturally sliding down. The li- mit knob (1) prevents accidental contact between specimen and objective. After locking the limit knob (by turning up and fastening) at a chosen height of the stage, you cannot focus the object closer to the objective using the coarse focusing knob. So, the object is protected against damage. (2) is the fine focusing knob.

Fig. 6 The convenient specimen holder (1) on the stage is used for holding the slide glass (2). The longitudinal (lengthwise) ad- justment knob (3) and the transversal (cross) adjustment knob (4) are coaxial. The stage moves expediently.

5. Adjustable Abbe condenser (Fig. 7)

Fig. 7 The condenser is moved up or down via turning the up-down knob (1). For centering, the condenser centering screws (6) are used. The condenser can be taken down easily if one un- screws the condenser holding screw (2). The place for a filter plate is on the filter seat (5).

6. Power switch and adjustable brightness (Fig. 7)

Turn down the power switch (3). Adjust the light control (4) until the image can be observed comfortably. Note: Don‘t let the light control at the position of maximum brightness for a long time. That reduces the life-span of the lamp.

The Köhler illumination is the optimal microscopic illumination and therefore standard for scientific research and micropho- tography. One gets it using the fixed field diaphragm and the height- and center-adjustable Abbe condenser: a) Using the condenser up-down knob (1), move the conden- ser (Fig. 3, No. 8) to the highest position, right under the stage. b) Turn on the power switch (3) and focus the object. c) Shut the field diaphragm (5) as close as possible. If the image of the field diaphragm lies out of the field of view, move it into the field using the condenser centering screws (6). d) Using the condenser up-down knob (1), change the height of the condenser, until the image of the field diaphragm is clear. e) Using the condenser centering screws (6), center the image of the field diaphragm in the field of view. f) Open the field diaphragm so widely, that its edge has only just left the field of view and this field is complete illumi- nated. It may be, that you have to center the condenser a little bit again. Now, adjust the aperture diaphragm, which is described in the next paragraph.

8. Aperture diaphragm (Fig. 8)

Fig. 8 The aperture diaphragm lever (Fig. 7, No. 4) can be turned in order to open or close the aperture diaphragm. Remove the eyepieces and watch through the eyepiece tube. For cente- ring, the condenser centering screws (Fig. 7, No. 6) are used when the diaphragm image is eccentric with the objective pupil (1). Turn the aperture diaphragm lever for getting a good resolution and contrast perception. Usually, the diameter of the aperture diaphragm image (2), which has to be adjusted, is 70-80 percent of the objective pupil.

9. Exchange of the lamp (Fig. 9)

a) Switch off the power switch and pull out the plugs of the AC-adapter from mains socket and from mains in at the microscope (Fig. 2, No. 7). b) Incline the microscope, loose the fixing screw (3) of the lamp door on the middle part of the bottom and open the lamp door; so, you remove the lamp baseboard (1) from the bottom. c) Pull out the old lamp (4) from the lamp base (5). Be careful, as the lamp may be hot! d) Insert the new lamp (4) into the lamp base (5). Notice the properly touching; take care not to touch the lamp with bare fingers. E. g., use the protective envelope of the lamp or a tissue, in order to grasp the bulb. e) Reinstall the lamp door (1) with lamp base board (5) on the bottom with the screw (3). f) After mounting the lamp well, plug in the power cord, turn on the power switch, turn the objective lens into the light path, adjust the condenser upwards and downwards, and make light enter the view field. If the light spot is offset from the center of view, loose the screw (2) slightly and move the lamp base (5). Move the lamp spot into the center, then tighten up the screw (2) immediately. Fig. 9

Sweep the lens by lens tissue or soft fabric immersed with a mixed liquid of alcohol/ether. Clean the 100x oil objective from oil whenever you finish operating.

2. Clean the painted parts

The dust on the painted parts can be removed by gauze. For the grease spots, the gauze immersed slightly with aviation gasoline is recommended. Do not use organic solvents such as alcohol, ether or other thinner etc. for cleaning the painted parts or plastic components.

3. Avoid disassembling the microscope

Because of being a precise instrument, do not disassemble the microscope casually. That may cause serious damage to its performance.

Cover the microscope with the dust cover (made of po- lymethylmethacrylate or polyethylene) and place it there, where it is dry and mouldless. We suggest the storage of all objectives and eyepieces in a closed container with drying agent. B C 70-80 %

DECF11 EEC CONFORMITY ExPLANATION Bresser GmbH, resident in 46414 Rhede/Westf., Guten- bergstr. 2, Germany, explains the agreement with in the following specified EEC guidelines for this product: EN 61326: 1997 EN 61000-3-2 Product description: Biological microscope Model: BRESSER BioScience Rhede, July 20, 2007 Bresser GmbH Helmut Ebbert Managing director Warranty & Service The regular guarantee period is 2 years and begins on the day of purchase. To benefit from an extended voluntary guarantee period as stated on the gift box, registration on our website is required. You can consult the full guarantee terms as well as information on extending the guarantee period and details of our services at www.bresser.de/warranty_terms. Would you like detailed instructions for this product in a particular language? Then visit our website via the link below (QR code) for available versions. Alternatively you can also send an email to manuals@bresser.de or leave a message on +49 (0) 28 72 – 80 74-220*. Please always state your name, precise address, a valid phone number and email address, as well as the article number and name. *Number charged at local rates in Germany (the amount you will be charged per phone call will depend on the tariff of your phone provider); calls from abroad will involve higher costs. http://www.bresser.de/5750500