TL RC - Appareil photo sans miroir LEICA - Free user manual and instructions

USER MANUAL TL RC LEICA

MICROSYSTEMS Contents

Page Overviews Safety concept 4 Symbols

Safety regulations .

Relief contrast Path-folding mirror .. IsoPro'M cross stage Light intensity and color temperature USB mouse Methods with transmitted light . Relief display . Filter

Lamp replacement 16 Care, maintenance 17 Expansion diagram 18 Scope of delivery 19 Dimensioned drawing 20 Technical data 22

Transmitted-light bases TL RCTM and TL RCITM — Safety concept

Thank you very much for your trust in Leica Microsystems. We hope your work with our high-quality, efficient products is both enjoyable and successful.

In developing our instruments, we have placed great weight on simple, self-explanatory operation. However, take your time to read the user manual, in order to familiarize yourself with the optimal use of all the benefits and options of your stereo micro- scope. Should you have any questions, please consult your local Leica representative. You will find the address of the clos- est local representative as well as valuable information about products and services from Leica Microsystems on our home- page at www.leica-microsystems.com

We are gladly at your service. CUSTOMER SERVICE is a big thing with us. Not only before the sale, but afterwards as well.

Leica Microsystems (Switzerland) Ltd. Stereo & Macroscope Systems

www.stereomicrosco) y.com The user manual

This user manual is available in 20 additional languages on our interactive CD-ROM.

User manuals and updates are also available for download on our homepage at www.stereomicroscopy.com.

The user manual at hand describes the safety instructions,

assembly, and handling of the TL RCTM and TL RCITM transmit- ted-light bases.

Transmitted-light bases TL RCTM and TL RCITM — Safety concept

The TLRCTM/TL RCIM transmitted-light bases come with an interactive CD-ROM containing all relevant manuals in 20 additional languages. Keep this CD-ROM in a safe place, where it is available to the user. User manuals and updates are also available for download and print-out on our homepage at www.stereomicroscopy.com.

The TL RCTM and TL RCIM transmitted-light bases are a module of the Leica M stereo microscope series. This user manual describes the special functions of the transmitted-light base and con- tains important instructions for operating safety, maintenance and the accessory parts.

The M2-105-0 user manual for the Leica M stereo microscopes contains additional safety rules for the stereo microscope, accessories and electrical accessories, as well as instructions for mainte- nance.

You can combine the transmitted-light base TL RCTM with any cold light source and a glass fiber light guide {active f = 10mm, tail tube f = 13mm). Please read the user manual and the safety requirements ofthe supplier.

À Before assembly, start up and use, please read the user manuals cited above. In particular, please observe all safety instructions.

To maintain the unit in its original condition and to ensure safe operation, the user must follow the instructions and warnings contained in these user manuals.

Transmitted-light bases TL RCTM and TL RCITM — Safety concept

This symbol indicates information, which must be

read and obeyed. Disregarding these instructions

— can cause hazards to personnel!

— can lead to functional disturbances and damaged instruments.

VAN Warning of dangerous electrical voltage

This symbol indicates especially important

information, which must be read and obeyed

Disregarding these instructions can lead to

— can cause hazards to personnel!

— can lead to functional disturbances and damaged instruments.

VAN Warning - hot surface This symbol warns you of tangible, hot points, such as light bulbs.

CO I Important information

This symbol indicates additional information or explanations that intend to provide clarity.

Action D This symbol within the text indicates that certain operations must be carried out.

Explanatory notes + _ This symbol indicates additional notes and explanations provided in the text.

(1.5) Numbers in brackets within the descriptions relate to the figures and the items within those figures. Example (1.3): Figure 1 is located, for example, on Page 8 and item 3 is the filter carrier.

Transmitted-light bases TL RCTM and TL RCITM — Safety concept

1.2 Safety regulations

The transmitted-light bases TL RCTM and TL RCITM

fulfill the highest requirements for observation

and documentation of Leica stereo microscopes

ofthe M series. It contains a path-folding mirror,

a device for partial pupil illumination and relief

contrast generation, a ground-glass screen, an

additional condenser and Fresnel lenses. The

complete stand consists of:

— Transmitted-light base TL RCTM or TL RCITM

— Stand 300 mm or 500 mm long with focusing drive, manual coarse/fine or motorized focus

— Glass stage plate, clear, 220x170x4mm

— Light source and light guide according to requirements

— Leica MATS Thermocontrol System with heating stage

— Polarization set and many more (see Expansion diagram)

TL RCM and TL RCIM transmitted-light bases are used in the assembly of Leica M series stereo microscopes via tripod column and microscope carrier. They can be combined with any cold light source and light guide and allows you to observe transparent specimen with relief contrast and direct transmitted light. The TL RCITM transmitted- light base posseses an integrated halogen lamp that particularly supports control by Leica Appli- cation Suite (LAS) software.

If you use the transmitted-light base

TLRCTM/TL RCIM, its components, and the acces- sories in a way other than that described in the user manual, harm may be caused to people or objects.

— Change, rebuild or take apart parts, if not specifically instructed to in this manual.

— Let non-authorized persons open parts of the instrument.

— Use the transmitted-light base TL RCTM/TL RCITM for investigations and operations on the human eye.

The units or accessory components described in the user manual have been checked for safety and possible dangers. Always consult the respon- sible Leica office or the main plant in Wetzlar at any time there is a need to adjust the unit oritis combined with non Leica components which go beyond the scope of this manual.

Unauthorized adjustment of the unit or improper use renders the warranty null and void.

— Only use the transmitted-light base TLRCTN/TL RCITM in closed, dust free rooms and between +10°C and +40°C. Make sure that the rooms are free of oil vapors or other chemi- cal vapors and extreme humidity.

— Setup the electrical components at least 10 cm from walls and combustible objects.

— Avoid large temperature fluctuations, direct sunlight and vibrations. This can cause faulty measurements or microphotographs.

— In warm, or warm and humid climatic zones, the transmitted-light base TL RCTM/ TL RCITM requires particular care to prevent formation of fungus.

6 Transmitted-light bases TL RCTM and TL RCI" — Safety concept

Responsi s of person in charge of the

— The transmitted-light base TL RCTM/ TL RCITM and the accessories are only operated, main- tained and repaired by authorized and trained personnel.

— Personnel who use the instrument have read and understood this user manual and in partic- ular the safety instructions and obey them.

Repairs, servicing work

— Repairs may only be carried out by servicing engineers trained by Leica Microsystems or your own authorized technical personnel.

— Only original Leica Microsystems parts may be used.

— Before opening the devices, turn off power and unplug mains cables.

VAN If you touch the electric circuit of the equipment, you may receive an electric shock.

Transport — Use the original packaging for shipping or

transporting the transmitted-light base TL RCTM/

TLRCITM and the accessory components.

— To prevent damage through vibrations, remove and pack specially all the moving components which you installed yourself, in accordance with the user manual.

Transmitted-light bases TL RCTM and TL RCITM — Safety concept

Integration in third-party products

The following must be observed if Leica products are built into third-party products: The manufac- turer of the complete system or the person putting it on the market is responsible for adhering to applicable safety regulations, laws and guide- lines.

The products described here must be disposed off in accordance with applicable local laws and reg- ulations.

Adhere to general and local regulations relating to accident prevention and environmental pro- tection.

EU declaration of conformity

The transmitted-light base TL RCTM/ TL RCIM and the accessories have been constructed according to the state of the art and issued with an EU dec- laration of conformity.

1 Cooling element of integrated halogen illumination (TL RCIM only) 2 Adapter plate for easy assembly of focusing drives

3 Standard stage 10 447 269

4 Filter bracket for up to three filters

5 Control button for the upper and lower Rottermann-ContrastsTM flap 6 Button to rotate and side-to-side movement of mirror

7 TLRCIMtransmitted-light base

8 Transmitted-light bases TLRCTM and TL RCIM

Fig. 2 Adapter plate vertical pole -transmitted-light Fig. 3 Adapter between focusing drive and base microscope carrier

Fig.4 Back side of transmitted-light base TLRCIM Fig. 5 USB mouse for TL RCI control

6 Screws for halogen lamp replacement

Transmitted-light bases TLRCTM and TL RCIM A

A Before unpacking, make sure no person can get injured by falling or tilting part.

3.1 Unpacking the base

The base is delivered with the adapter plate attached. The selected stage (IsoProïM cross- stage or standard stage 10 447 269), and the focusing drive will have to be mounted later. Make sure the devices are unpacked on a flat, sufficiently dimensioned, and non-slip surface.

The TL RCTV/ RCITM transmitted-light base can be equipped with two different stages. The selected stage is mounted on the base before start-up. The two stages can be easily exchanged at any time.

The following paragraph assumes the base with- out the stage mounted. Disassembly is performed in reverse order of the following steps.

3.2.1 Standard stage

» Take the glass plate from the rectangular gap in the standard stage.

» Position the stage on the transmitted-light base in such way that the four holes align over those in the base.

» Attach the stage to the base with the four sup- plied Allen screws.

» Insert the glass plate back into the standard stage.

3.22 IsoPro" cross-stage

Before the IsoProT" cross-stage is mounted to the base, the axis containing the control buttons is attached optionally on the left or the right side of the cross-stage.

Ifthe controls shall be mounted on the left side, the gear rod on the bottom side of the cross-stage must be unscrewed and re-attached in reverse.

» Take the glass plate from the cross-stage.

» Turn the cross-stage around and place it onto a non-slip surface.

> Change the gear rod (6.2) from the left to the right side.

» Skip the next two steps to mount the controls.

» Take the glass plate from the cross-stage.

» Turn the cross-stage around and place it onto a non-slip surface.

» Attach the axis with the control buttons (6.1) to the desired side. The fastener snaps into the cross-stage magnetically.

» Attach the axis with the two supplied Allen screws.

» Attach the cover rail to the cross-stage.

Cross-stage assembly

» Place the cross-stage onto the base.

> Pull the upper part of the cross-stage carefully toward the user, fixing the lower part onto the transmitted-light base.

» Attach the cross-stage evenly to the three threaded holes.

» Push the cross-stage all the way back to the fence in direction of the column.

> Re-insert the glass plate back into the standard stage.

10 TLRCM and TL RCI" transmitted-light bases — Assembly

3.3 Focusing drive column

» Unscrew the adapter plate (1.2) from the base using the supplied Allen key.

» Attach your focusing drive column to the bottom with the three Allen screws (2).

» Re-attach the adapter plate to its original position.

3.4 Intermediate adapter assembly

Mount the supplied adapter between the column

and microscope carrier, to balance the enlarged

distance between focusing drive and optical axis.

» Attach the adapter (3) to the focusing drive for the pins to lock in recess.

» Attach the adapter using the supplied Allen key.

3.5 Equipment assembly

After mounting the adapter to the focusing drive, the microscope carrier, optics carrier, and the entire equipment can be assembled as usual.

3.6 Connecting the cold light-source to the TL RCTM base

» Push the appropriate end of the cold light guide into the base back-side.

» For further information about the use of cold light sources refer to the instructions supplied separately.

3.7 Connecting the power cable (TLRCIM)

»> Make sure the power switch (4.1) on the base is turned to ,0".

» Insert the power cable into the mains socket (4.2), and then connect it to a grounded outlet.

Fig 6 Rear side of the IsoPro!" cross-stage

1 Axis with controls 2 Gear rod, mounted to cross-stage

Fig. 7 Cross-stage controls

1 Control button for x-direction movement 2 Control button for y-direction movement

TLRCTM and TL RCIMtransmitted-light bases — Configuration

4.1 Switch for adjusting the relief contrast

Use the two switches (8.1 and 8.2) on the left side ofthe transmitted-light base TL RCTM/TL RCITM to actuate the two integrated flaps. The outer switch 18.1) controls the inverted relief contrast. The inner switch (8.2) controls the positive relief con- trast. Depending on the flap position, part of the opening of the integrated Fresnel lens is covered causing different contrast effects. Phase struc- tures typically appear as spatial, relief images — like hills in a positive relief contrast and as recesses in inverted relief contrast.

The contrast can be increased without relief by adjusting the two diaphragms to 45°. A slot shaped illuminated area appears. The slot can be moved over the entire field of view and quickly switched between positive and negative relief view by precise tilting of the path-folding mirror. The dynamic effect makes it simple to distinguish phase structures from amplitude structures.

CE Depending on the properties of the object (refractive index to environment) and your own it may occur that you need to operate hes for the positive and inverted relief contrast in reverse. These switches are described below.

Le. instead of the top switch (8.1), the bottom switch (8.2) controls the inverted relief contrast. Instead of the bottom switch (8.2), the top switch (8.1) controls the positive relief contrast.

4.2 Path-folding mirror

The built-in path-folding mirror features a flat and a concave side and can be rotated and moved. The concave side was specifically designed for the optical features of objectives with high

numeric aperture. Using the black rotary knob (8.1) on the left side of the transmitted light base you can turn and push the path-folding mirror back and forth.

The concave recess atthe grip shows the con- cave side of the mirror and allows the intuitive operation without eye contact at anytime.

= I Depending on the angle and position of the mirror the angle of light incidence changes on the specimen level so that you can move between

a transmitted light bright field, through oblique illumination to dark field like illumination.

The black rotary knob (8.1] on the left side of the

transmitted-light base is used:

— To rotate the path-folding mirror from the flat to the concave side

— For slight tilting, to steer the light beam more steeply or flatly through the object level

— To shift the path-folding mirror (forward/ backward)

43 Operating the cross-stage IsoPro'M D> To move the stage in X direction rotate the outer knob (7.1)

D> To move the stage in Y direction rotate the inner ring around the knob (7.2)

4.4 Light intensity and color temperature 4.4.1 Transmitted-light base TL RCTM

À Please observe the user manual and in particular the safety regulations of the manufac- turers of the light guide and cold light source. D» Turn on the cold light source according to the manufacturer's user manual and connect, activate, and adjust the light intensity.

12 TLRCM and TL RCI" transmitted-light bases — Operation

Fig. 8 1 Switch to control the inverted relief contrast

2 Switch to control the positive relief contrast 3 Path-folding mirror

4.4.2 TL RCI transmitted-light base

The TL RCITM transmitted-light base has two elec- tronic potentiometers to control the dye intensity (9.1), and dye temperature (9.2) separately.

» Adjust the dye intensity using the front poten- tiometer (9.1) to match your requirements.

> With the rear potentiometer (9.2), setthe dye temperature to match the color impression to your requirements.

The dye temperature control also serves as

» For process interruption, press the poten- tiometer (9.2).

» To switch on the illumination, press the poten- tiometer again. The electronics return to the previously selected settings.

1° Potentiometer Control of IsoColM" illumination intensity 2 Potentiometer Control of dye temperature

4,5 Leica USB mouse (TL RCITM only)

The Leica USB mouse controls the IsoCol'" and the dimming function of the TL RCITM base

> Connect the mouse (5) to the appropriate USB port of the base (4.4).

+ By default, the scroll wheel ofthe mouse is assigned to the IsoCol'" control.

> Scroll away from the user to increase the illumination intensity in IsoCol"M mode.

> Scroll towards the user to decrease the illumination intensity in IsoCol"M mode.

» Click the scroll wheel briefly to switch the illumination on or off.

» To switch between the IsoColM- and dimming mode and back, click on and hold the scroll wheel for more than 2 seconds.

+ The intensity in the dimming mode changes analog to the IsoCol'M mode.

TLRCM and TL RCI" transmitted-light bases — Operation 13

4.6 Methods with transmitted light

4.6.1 Vertical bright field illumination

Suitable for colored amplitude preparations with Sufficient contrast.

> Both switches should be placed in horizontal position.

+ The flaps are open.

D Using the black rotary knob (8.3) on the left- hand side of the transmitted-light base, shift the path-folding mirror towards the column until it hits the stop.

D According to the aperture of the used objective rotate either the plane or concave side ofthe path-folding mirror toward the top.

> Turn the path-folding mirror to the notch position at 45°.

The light beams are steered vertically through the object. The outcome of this is an exact bright field with maximum brightness.

4.6.2 Inclined transmitted light

Suitable for semitransparent, opaque objects such as foraminiferes and fish eggs.

> Both switches should be placed in horizontal position.

+ Theflaps are open.

D Turn the path-folding mirror (8.3) so thatthe light beams fall through the object diagonally.

D Shiftthe path-folding mirror until the required information is visible.

4.3 Asymmetrical dark-field illumination

> Both switches should be placed in horizontal position.

+ Theflaps are open.

D Rotate the path-folding mirror (8.3) so thatthe light beams pass flatly through the object.

The flatter the light rays are deflected into the objects the darker the background appears. Atransmitted light similar to a dark field is cre- ated. Contours, fine edges and structures are brightly emphasized by diffraction of the light onthe dark background.

D Using the black rotary knob (8.3) on the right- hand side of the transmitted-light base, shift the path-folding mirror towards the column until it hits the stop.

D» Turn the path-folding mirror to the notch position at 45°.

4.6.1 Positive relief contrast

> Both switches should be placed in horizontal position.

+ _Theflaps are open.

D Rotate the inner switch (8.2) close to the upright position.

+ The bottom flapis closed. There is positive relief contrast. The phase structures act like hills.

D Intensify or weaken the effect by tilting the path-folding mirror.

4.6.2 Negative relief contrast

D Rotate the inner switch (8.2) close to the horizontal position.

+ The bottom flap is open.

D> Rotate the upper switch (8.1) close to the upright position.

+ Thetopflapis closed. There is negative relief contrast. Phase structures appear as recesses.

D Intensify or weaken the effect by tilting the path-folding mirror (8.3).

14 TLRCM and TL RCI" transmitted-light bases — Operation

4.6.3 Dynamic relief contrast

D Place both switches in about a 45° degree

The flaps are set to 45°. A slot shaped illumi- nated area appears. The slot can be moved over the entire field of view and can be quickly switched between positive and negative relief view by precise tilting of the path-folding mirror (8.3). The dynamic effect makes it simple to distinguish phase structures from amplitude structures.

The relief methods give good results from medium zoom up to a high level of magnification and with the 1x, 1.6x and 2x objectives. In the bottom half ofthe zoom scale and with weaker objects, it may occur that the object field is not homogeneously lit. We recommend using the transmitted-light base with objectives of 1x and higher and not with objectives with long focal distances.

Fig. 10 Switch positions to control the transmitted light

Switch position with transmitted light: Both flaps are open

Switch position with positive relief contrast, medium

Switch position with positive relief contrast, strong

Switch position with inverted relief contrast, medium

Switch position with inverted relief contrast, strong

Switch position with increased contrast without relief contrast

Switch position with increased contrast without relief contrast, path-folding mirror tilted Switch position with increased contrast without relief contrast, path-folding mirror tilted

CREME ENCRES TLRCM and TL RCI" transmitted-light bases — Operation 15

TLRCIM and TL RCIM transmitted-light bases can be equipped with up to three filters simultane- ously, that are available as accessory (see Expan- sion Diagram page 18/19). The individual filters are also available to customers upon special request.

D» Turn off the light source or press (TL RCITM) the switch for the shutter (9.2).

D» Retrieve the empty filter from an available filter location at the filter holder.

D Insert the desired filter.

D» Activate the light source again.

4.8 Changing the halogen lamp (TL RCITM)

VAN Itis absolutely necessary that you unplug the power cord form the base before changing the lamp in order to prevent possible electrical shock!

À The halogen lamp is getting very hot during operation. Keep the base turned off for about

10 minutes to allow for cooling and in order to prevent burns!

> Loosen the two bolts at the cooling element (1.1).

> Carefully pull out the entire cooling element, including the lamp.

D Remove the lamp holder (11.2) by pulling it from the guide rail.

> Carefully pull off the lamp including its socket from the holder.

D> Remove the lamp (11.3) out of the socket.

FI I Never touch the new halogen lamp with bare fingers since this will significantly reduce the life expectancy of the lamp!

D Push the new lamp into the socket. > Perform all above identified steps in reverse order.

L Fig. 11 TL RCITM transmitted-light base open lamp housing

1 Screws for loosening the cooling fins

16 TLRCTM and TL RCIMtransmitted-light bases — Operation

We guarantee the quality

Protect your instruments

we would like to explain and help you understand the careful handling of your valuable instrument, and provide you with some tips for proper maintenance and cleaning.

You are using a precision instrument with many performance features.

As appropriate for such instruments we guarantee the quality of our products. This guarantee covers all manufacturing and material defects of the al equipment, but not any dam- ages that are the result of inappropriate use or negligence.

Please handle your valuable optical instrument with appropri- ate care. If you do so you will be able to enjoy many decades of accurate service by this equipment without any deterioration. This is the reputation of our equipment.

Should your equipment, however, no longer provide accurate service, please contact your authorized service representative, your Leica distributor or directly

Leica Microsystems (Switzerland) Ltd., CH-9435 Heerbrugg.

*_ Protect from moisture, fumes, acids, bases and corrosive materials. Do not store any chemicals in close proximity of the instrument.

Protect from inappropriate use or handling. Never use off-brand electrical connectors or wires; never disassemble optical sys- tems or mechanical components, if no particular reference is made to that pur- pose in the instructions.

Protect from oil and grease. Slide surfaces and mechanical components shall never be lubricated.

TLRCTM and TL RCIMtransmitted-light bases — Care, Maintenance 17

18 TLRCM and TL RCI" transmitted-light bases — Expansion diagram

Incident-light bases Incident-light base for S series Transmitted-light sub-base for incident-light base S series Incident-light base for M series Transmitted-light base TL ST Transmitted-light base TL BFDF Transmitted-light base TL RCTM for external cold light sources TLRCITMtransmitted-light base with integrated halogen illumination

Standard stage for transmitted-light bases TL BFDF, TL RCTM and TL RCITM Cross-stage for transmitted-light bases TL BFDF, TL RCTM, TL RCITM

and incident-light base (with adapter 10 447 368)

Adapter between cross-stage

and incident-light base 10 447 342

Thermo stage Leica MATS TL with controller

Adapter for stages with 120mm

Stage for LifeOnStage accessory

Universal carrier for Petri inserts,

object carriers (up to four pieces) etc.

Column adapter for micromanipulation

Sliding stage, 2120mm Polarization stage, 2120mm Objective guide for polarization stage

Compensator Red | for Pol rotating stage

Universal carrier, 2120mm

Glass insert with Pol, Z120mm

Focusing drive with 300mm profile column for incident and transmitted- light bases

Focusing drive with 500mm profile column for incident and transmitted- light bases

Focusing drive, fast/fine, with 300mm profile column for incident and transmitted-light bases Focusing drive, fast/fine, with 500mm profile column for incident and transmitted-light bases Motorized focus drive with 300mm column and power adaptor for inci- dent and transmitted-light bases Motorized focus drive with 500mm column and power adapter for inci- dent and transmitted-light bases

Day light filter for basis TL ST BG38 Fluorescent filter for transmitted-light bases TL RCTM/

RCITM UV filter for bases TL RCTM/ RCITM Filter ND (gray filter] for bases

TLRCTM/ RCITM Illumination

Leica USB mouse, freely assignable five key mouse for connecting to transmitted-light bases TL RCITM

or PC USB cable to connect TL RCITM basis

to a PC Foot switch with CAN bus connector

Ergo accessories Leica ErgoRest (palm rest to prevent tiresome work environment]

TLRCM and TL RCI transmitted-light bases — Scope of delivery 1

Measurements transmitted-light base TL R(

Transmitted-light base TL RCTM Transmitted-light base TL RCTM with standard stage 10 447 269 with IsoProM cross-stage

20 Transmitted-light bases TL RCTM and TL RCITM - Technical data

Measurements TL RCI" transmitted-light base

TLRCITM transmitted-light base with TLRCITM transmitted-light base with standard stage 10 447 269 IsoProïM cross-stage

Transmitted-light bases TL RCTM and TL RCITM - Technical data

Fast exchange of illuminant Illuminated area Power Connector

Weight Illumination types

{Constant Color Intensity Control) Internal shutter/Lamp control Integrated filter holder

raise color temperature Matching of high num. aperture Remote control possi AntiShockM Pads Size ofthe base (WxHxD, in mm)

for outstanding products

Leica Microsystems’ mission is to be the world first-choice provider of innovative solutions to our customers’ needs for vision, measurement, ithography and analysis of microstructures.

Leica, the leading brand for microscopes and scientific instruments, developed from five brand names, all with a long tradition: Wild, Leïtz, Reichert, Jung and Cambridge Instruments. Vet Leica symbolizes innovation as well as tradition.

Leica Microsystems — an international company with a strong network of customer servic

Australis Gladesvile, NSW Tel. +1800625286 Fax +61 2 9817 8358 Austria Vienna Tel. +43 1486 80 500 Fax +43 1 486 80 50 30 Canada: Richmond Hil/Ontario Tel. +1 905 762 20 00 … Fax +1 905 762 89 37 China: Hong Kong Tel. +852 5646699 Fax +8522 564 4163 Denmark Herlev Tel. +45 44 5401 01 Fax +45 44 5401 11 France: Rueil-Malmaison

Cédex Tel. 433147328585 Fax +33 1 4732 8586 Germany: Bensheim Tel. +49 62511360 Fax +49 6251 136 155 Mely: Tel. +39 02 57 486 1 Fax +39 02 5740 3273 Japan: Tel. +813 543 596 09 Fax +81 3 543 596 15 Korea: Tel. +8225146543 Fax +82 2 514 6548 Netherlands: Tel. +31 70 4132130 Fax +31 70 41 32 109 Portugal: Lisbon Tel. +35 1213814 766 Fax +35 1 213 854 668 Singapore: Tel. 6567797823 Fax +656 7730628 Spain Barcelona Tel. +34 93 494 9530 Fax +34 93 494 9532 Sweden: Sollentuna Tel. +4686254545 Fax +46 8 625 45 10 Switzerland: Glattbrugg Tel. +41 44 809 34 34 Fax +41 44 809 34 44 United Kingdom: Miton Keynes Tel. +44 1908 246 246: Fax +44 1908 609 992 USA: Bannockbur/Ilinois Tel. +1 800 248 0123 Fax +1 847 405 0164

and representatives of Leica Microsystems in more than 100 countries.

In accordance with the ISO 9001 certificate, Leica Microsystems (Switzerland) Ltd, Business Unit Stereo & Macroscope Systems has at its disposal a management system that meets the requirements of the international standard for quality management. In addition, production meets the requirements ofthe international standard ISO 14001 for environmental management.

Leica Microsystems (Switzerland) Ltd Stereo & Macroscope Systems CH-9435 Heerbrugg

Telephone +41 71 726 33 33 Fax +41 71 726 33 99 wwwleica-microsystems.com wwwstereomicroscopy.com

The companies of the Leica Microsystems Group operate international in four business: segments, where we rank with the market leaders.

+ Microscopy Systems Our expertise in microscopy is the basis for all our solutions for visualization, measurement and'analysis omicrestructures in fe sciences and industry. With confocal laser technology andimage analysis systems, we provide thre- dimensional viewing facilities and offer new solutions for eytogenetcs, pathology andmate- ral sciences.

+ Specimen Preparation We provide comprehensive systems and ser- vices for clinical Misto- and cytopathology applications, biomedical research and indus- trial quality assurance. Our produet range includes instruments, systems and consum- ables for tissue infitration and embedding, micratomes and cryostats as well as auto- mated staners and coversippers.

Innovative technologies in our surgical micro- Scapes offer new therapeutic approaches in mierosurgery,

+ Semiconductor Equipment Our automatedleading-edge measurementand inspection systems and our E-beam ithography systems make us the first choice super for semiconductormanufacturers all overthe word

strations, descriptions and technical data are not binding and may be changed without notice.

M2-218-2en+ © Leica Microsystems Switzerland} Lid+ CH-9435 Heerbrugg, 2005 + Printed in Switzerland — en - XI1.2005 - RDV