EG1150 C - Microtome LEICA - Notice d'utilisation et mode d'emploi gratuit

Intitulé	Description
Type de produit	Microtome à cryostat
Caractéristiques techniques principales	Précision de coupe, température réglable, système de réfrigération intégré
Alimentation électrique	220-240 V, 50/60 Hz
Dimensions approximatives	Largeur : 60 cm, Profondeur : 70 cm, Hauteur : 50 cm
Poids	Environ 70 kg
Compatibilités	Compatible avec divers types de blocs de paraffine et de tissus biologiques
Type de batterie	Non applicable
Tension	220-240 V
Puissance	Environ 300 W
Fonctions principales	Coupe de sections fines de tissus, conservation à basse température
Entretien et nettoyage	Nettoyage régulier des lames et des surfaces, vérification du système de réfrigération
Pièces détachées et réparabilité	Disponibilité de pièces de rechange, service après-vente recommandé pour les réparations
Sécurité	Utiliser des gants lors de la manipulation des lames, respecter les consignes de sécurité électrique
Informations générales utiles	Idéal pour les laboratoires de recherche et d'histologie, formation recommandée pour les utilisateurs

FOIRE AUX QUESTIONS - EG1150 C LEICA

Comment allumer le microscope LEICA EG1150 C ?

Pour allumer le microscope, localisez le bouton d'alimentation sur le panneau avant et appuyez dessus. Assurez-vous que l'appareil est correctement branché.

Que faire si l'image est floue ?

Vérifiez l'objectif pour vous assurer qu'il est propre. Ajustez la mise au point en tournant la molette de mise au point. Assurez-vous également que l'échantillon est correctement placé sur la platine.

Comment changer l'objectif ?

Pour changer l'objectif, tournez doucement l'objectif actuel pour le dévisser, puis vissez le nouvel objectif en place. Assurez-vous de sélectionner un objectif compatible avec le modèle EG1150 C.

Quel type d'échantillons puis-je utiliser avec le LEICA EG1150 C ?

Le LEICA EG1150 C est conçu pour des échantillons biologiques et histologiques, tels que des coupes de tissus, des cellules et des préparations microscopiques.

Comment régler l'éclairage du microscope ?

L'éclairage peut être ajusté à l'aide du variateur d'intensité situé sur le panneau de contrôle. Tournez le variateur pour augmenter ou diminuer la luminosité selon vos besoins.

Que faire si le microscope ne s'allume pas ?

Vérifiez que le câble d'alimentation est correctement branché à la prise murale et au microscope. Testez la prise avec un autre appareil. Si le problème persiste, contactez le support technique.

Comment nettoyer les optiques du microscope ?

Utilisez un chiffon doux et non pelucheux, légèrement imbibé d'alcool isopropylique pour nettoyer les lentilles. Évitez d'utiliser des produits chimiques agressifs qui pourraient endommager les surfaces.

Le logiciel de contrôle du microscope est-il inclus ?

Oui, le LEICA EG1150 C est généralement livré avec un logiciel de contrôle qui vous permet de capturer et d'analyser des images. Veuillez vérifier le contenu de votre emballage pour plus de détails.

Quelle est la garantie offerte avec le LEICA EG1150 C ?

Le LEICA EG1150 C est généralement couvert par une garantie de 2 ans. Veuillez consulter les documents fournis avec votre appareil pour les conditions spécifiques.

MODE D'EMPLOI EG1150 C LEICA

Exterior view of a laboratory instrument labeled 'Jelica 2016' with control panel and display screen (no readable text beyond branding)

Leica EG1150C

Cold Plate

CE

Instruction Manual

Leica EG1150C

V2.1 Rev B, English – 01/2010

Always keep this manual near the instrument.

Read carefully prior to operating the instrument.

Leica

MICROSYSTEMS

The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state-of-the-art technology as we understand it following thorough investigation in this field.

We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual.

For erroneous statements, drawings, technical illustrations etc. contained in this manual we exclude liability as far as permissible according to the national legal system applicable in each individual case. In particular, no liability whatsoever is accepted for any financial loss or consequential damage caused by or related to compliance with statements or other information in this manual.

Statements, drawings, illustrations and other information as regards contents or technical details of the present manual are not to be considered as warranted characteristics of our products.

These are determined only by the contract provisions agreed between ourselves and our customers.

Leica reserves the right to change technical specifications as well as manufacturing processes without prior notice. Only in this way is it possible to continuously improve the technology and manufacturing techniques used in our products.

This document is protected under copyright laws. Any copyrights of this document are retained by Leica Biosystems Nussloch GmbH.

Any reproduction of text and illustrations (or of any parts thereof) by means of print, photocopy, microfiche, web cam or other methods – including any electronic systems and media – requires express prior permission in writing by Leica Biosystems Nussloch GmbH.

For the instrument serial number and year of manufacture, please refer to the name plate at the back of the instrument.

© Leica Biosystems Nussloch GmbH

Issued by:

Leica Biosystems Nussloch GmbH

Heidelberger Str. 17 - 19

D-69226 Nussloch

Germany

Phone: [phone]

Fax: [phone]

Internet: http://www.leica-microsystems.com

1. Important notes .... 5

1.1 Symbols used in the text and their meanings 5
1.2 User group 5
1.3 Specified use and application 5
1.4 Instrument type 5

2. Safety 6

2.1 Safety regulations....6
2.2 Safety instructions 6
2.3 Built-in safety devices 7

3. Instrument components and specifications 8

3.1 Overview – instrument parts 8
3.2 Technical data 9

4. Commissioning 10

4.1 Location conditions 10
4.2 Delivery range 10
4.3 Unpacking and installing 11
4.4 Power supply 12

5. Operation 13

5.1 Switch on the instrument 13
5.2 Replacing the secondary fuse 13
5.3 Malfunctions 14

6. Cleaning and maintenance 15

6.1 Cleaning the instrument.... 15
6.2 Maintenance instructions 15

7. Warranty and service 16

1.1 Symbols used in the text and their meanings

Warnings and cautions appear in a gray box and are marked by a warning triangle ⚠️.

Notes, i.e. important information for the user, appear in a gray box and are marked with the symbol i.

(5)

Figures in brackets refer to item numbers in figures.

1.2 User group

• The Leica EG1150C may only be operated by qualified personnel.
• The user must read the operating instructions supplied and be familiar with all the instrument's technical details before any work on the instrument can be carried out.

1.3 Specified use and application

The Leica EG1150C is a cold plate for chilling and blocking out histological tissue samples in paraffin blocks.

Any other use of the instrument will be considered as improper use!

1.4 Instrument type

All information in this instruction manual applies only to the instrument type indicated on the title page.

A name plate with the serial number is attached to the back of the instrument.

Leica ! Modell Fabr.Nr. 1681/06.2002 EG 1150 C-11 Kat.-Nr. 038831681 100 V~ 50/60 Hz 300 VA Kältemittel R134a Fullmenge 80 ± 5g Maximal zulässiger Betriebsdruck 18 bar SF 3 Leica Microsystems Nussloch GmbH D-69226 Nussloch Vog en Germany

Fig. 1

Please pay particular attention to the safety regulations and safety instructions in this section.

Please also read this information, even if you are familiar with handling and using a Leica instrument.

2.1 Safety regulations

This instruction manual contains important instructions and information regarding the operational safety and maintenance of the instrument.

The instruction manual is an important part of the product. It must be read carefully before using the instrument for the first time and always kept near the instrument.

It may be necessary to add appropriate instructions to this instruction manual, if required by existing national regulations on accident prevention or environmental protection in the country of the operating authority.

This instrument has been built and tested in accordance with the following safety regulations on electrical measuring, control, regulating and laboratory devices.

In order to maintain this condition and to ensure safe operation, the operator must observe the instructions and warnings contained in this instruction manual.

For current information about applicable standards, please refer to the CE declaration of conformity on our Internet site:

www.leica-microsystems.com

The protective devices on the instrument and its accessories must not be removed or modified. The instrument may only be opened and repaired by authorized Leica servicing technicians.

2.2 Safety instructions

The safety devices provided on this instrument by the manufacturer only constitute the basis for protection against accidents. The main responsibility for accident prevention while using the instrument rests with the employer where the instrument is operated, and with the persons he appoints for operating, servicing or cleaning the instrument.

To ensure a safe operation of the instrument at all times, the following instructions and warnings should be observed.

Safety instructions – safety regulations on the instrument itself

• This device may only be used by trained laboratory technicians. It may only be used according to its intended use and the instructions in this instruction manual.
• Safety regulations marked with a warning triangle on the instrument itself mean that when operating or exchanging respective parts of the instrument, the correct operating steps as described in the instruction manual supplied, must be adhered to. Nonobservance can cause accidents, injuries and/or damage to the instrument/accessories.

Safety instructions – transport, installation and operation

• After unpacking the instrument it may only be transported in an upright position.
- Before connecting the device to a power source, ensure that the voltage indicated on the type plate matches the voltage available at the place of installation.
The unit must be connected only with the supplied power cable and only to a grounded power receptacle. Do not use an extension cord.
- The minimum voltage (see specifications) must be maintained while starting the refrigeration unit.
The compressor needs a starting current of approx. 25 A. For correct operation, a consistent power supply conforming to the instrument's specifications is required. Please ensure that your electrical installation fulfills these preconditions prior to installing the unit.
Nonobservance causes damage to the instrument.
- Switch off the instrument each time before servicing, repairing or cleaning, and pull out the power plug.

2.3 Built-in safety devices

Short- circuit 0 1 0 1

Fig. 2

Automatic circuit breaker in the main switch

• An automatic circuit breaker with a lit switch is located in the main switch. This circuit breaker separates the unit from the mains power supply in the event of a short-circuit.
• In this case, the main switch jumps to the position "0" = 0ff.

3.1 Overview – instrument parts

• The instrument is distinguished by a simple, modular design and a powerful refrigeration unit with precisely controlled cooling performance.
• High temperature consistency ensures homogeneous blocking out and prevents tearing of the paraffin blocks.
• High cooling performance ensures that the instrument's working temperature is reached quickly.
• Optimized temperature distribution in the cold plate prevents dripping condensation.
• The generously-dimensioned cooling surface has room for around 70 blocks.
• Designed for use with the Leica EG1150H paraffin embedding station.

Illuminated on-off switch (automatic fuse) LEICA 1150C Refrigeration system (inside) Hinged hood (optional) Cooling surface Adjustable feet, front and back Fig. 3

3.2 Technical data

General specifications

Approvals:

The approval symbols relating to this instrument are located on the rear of the instrument next to the nameplate.

Voltage of power supply:

The device requires a specific AC voltage, see table at right. Instrument type acc. to identification label.

AC ±10 %

Instrument type | Voltage of power supply

EG1150 C-1	230 V / 50 Hz
EG1150 C-3	120 V / 60 Hz
EG1150 C-8	240 V / 50 Hz
EG1150 C-11	100 V / 50 - 60 Hz

Maximum input:

Maximum starting current (5 s):

Class of protection ^1 :

Mains power fuse 100/120 V:

Mains power fuse 230/240 V:

Miniature fuse 6.3 x 32 mm:

Degree of pollution ^1 :

Overvoltage category:

Operating temperature range:

Working temperature range:

Relative air humidity:

^1) according to IEC-1010, UL 3101, EN 61010

350 VA

25 A

|

ETA circuit breaker type 3120-F421-P7T1W19DB4-10A

ETA circuit breaker type 3120-F421-P7T1W19DB4-4A T 1.0 A, Schurter: type Fst

2

||

+18°C to +40°C

- 5 °C, up to a room temperature of approx. +28 °C

maximum 80 %, non-condensing

Refrigeration unit

Refrigeration performance*:

Safety factor:

Refrigerant:

Compressor oil:

*as per CECOMAF

Liquid temperature: 55°C

Evaporation temperature: -25 °C

110 W

3

80 g ± 5 g R 134a

180 cm ^3 Emkarate RL 15 s, ICI

Dimensions and weight

Width:

Depth:

Height:

Weight:

360 mm

650 mm

360 mm

23 kg

4.1 Location conditions

• Stable, vibration-free laboratory table with horizontal, flat table top, as far as possible vibration-free ground.
• No direct sunlight or strong temperature fluctuations. Room temperature throughout between +15 °C and +28 °C.

At a room temperature of > +28^ the working temperature of the cold plate of -5^ may not be reached at all points.

• Relative air humidity maximum 80%, non-condensing.
• The instrument should be set up in such a way that the air circulation is not affected.

To ensure proper function, there must be gap of at least 15 cm behind the instrument. Failure to observe this distance may result in serious damage to the refrigeration unit of the device. The instrument should not be operated in hazardous locations.

4.2 Delivery range

The basic equipment for the Leica EG1150C cold plate contains the following components:

Order No.

• 1 basic instrument, Leica EG 1150C
• 1 power cable see p. 12 only for Australia:
• 1 power cable EU 14 0411 13558
• 1 mains transformer, 230/240 V 14 0469 31998
• 1 replacement fuse T 1.0 A, 6,3x32 mm 14 6943 01001
• 1 operating manual 14 0388 80002
• optionally: protective hood (acrylic)

Please compare the delivered components against the packing list and your order.

Should there be any discrepancy, please contact the Leica distributor handling your order.

4.3 Unpacking and installing

The unpacking instructions are attached to the outside of the transport crate. Fig. 4 shows the design of the original packaging.

The numbers indicate the sequence of disassembling and reassembling.

Please check the transport packaging for external damage.

If you detect obvious damage, please report it to your shipping agent immediately.

1 3 2 2 4 5

To lift device, grasp at the front on the base plate and at the rear at the base of the housing (arrows in Fig. 5), lift out of the shaped pad (4) and place on a sturdy laboratory table.

The Leica EG1150C must be transported in an upright, horizontal position at all times. It must not be inverted under any circumstances, even for short periods, or stored on one of its sides.

A waiting period of 4 hours must be observed between transporting the instrument and switching it on. The compressor oil agitated while moving the instrument must return fully to its original position.

3D rendering of a beige LBCA ES INOC device with a blue base and green tray, labeled Fig. 5 (no text or symbols on the device itself)

4.4 Power supply

The refrigeration unit of the Leica EG1150C requires a specific voltage and frequency (see "Technical data" chapter) and is therefore supplied with an AC power cable suitable to the instrument.

Please observe the following notes to prevent damage to the unit:

Before connecting the device to a power source, ensure that the voltage indicated on the type plate (rear) matches the actual voltage available at the place of installation.

If this is not the case, do not plug the instrument in!

The unit must be connected only with the supplied power cable and only to a grounded power receptacle.

Do not use an extension cord!

• Connect the plug (7) of the power cord to the connecting port (6).
• Plug the power cord into the wall outlet.

Diagram showing a hand inserting a card into a device labeled with components 6 and 7, illustrating the process of inserting or removing a card.

Fig. 6

To connect the instrument, use one of the power cords listed below:

Location Order No.

Australia:	14 0411 32565
Europe:	14 0411 13558
Italy:	14 0411 13561
Switzerland:	14 0411 13560
United Kingdom:	14 0411 27822
USA, Canada, Japan:	14 0411 13559

5.1 Switch on the instrument

Green toggle switch with '0' and '1' labels (no additional text or symbols)

After the installation as described in Chapter 4 is complete, the Leica EG1150C is ready for use. Switch the instrument on with the on-off switch at the front left-hand side ("I" = ON). A lamp lights up in the switch to indicate that the unit is operational and the refrigeration unit will start working.

Depending on the room temperature, the working temperature of the cooling surface (-5 °C) will be reached in around 25 minutes (see also Ch. 4.1, p. 10).

The cooling surface may not be loaded with molds until the cooling time has elapsed. Otherwise, the working temperature of -5 ^ may not be reached.

5.2 Replacing the secondary fuse

LDA T80C 9 1 AT 10 9

Fig. 7

A miniature fuse to protect the electronic components is located on the rear of the instrument.

Before replacing the fuse, always switch the instrument off and pull the power plug from the wall socket. Use only miniature fuses of the type specified in the 'Technical data' chapter.

To replace the fuse, please proceed as follows:

• Using a screwdriver, lightly press the fuse holder (9) and turn counterclockwise about 14 turn.
• Remove the fuse holder and take out the fuse (10).
• Replace it with a new fuse of the same type.
• Replace the fuse holder (9).
• Using a screwdriver, gently press the fuse holder and turn it clockwise about 1 / 4 turn.
• Reconnect the instrument to an AC power outlet and switch it on.

5.3 Malfunctions

If you cannot solve your problem using the help in the following table, please contact your Leica customer service organization or the Leica dealer from whom you purchased the instrument.

Malfunction	Possible causes	Corrective action
Instrument doesn’t work.	Power switch not turned on or the cut-out function of the power switch activated.	Check instrument is connected, then turn main power switch on. Only for Great Britain: Check the fuse in the mains plug.
Cooling unsatisfactory, formation of condensation.	Room temperature too high or exposure to direct sunlight.	Check the cooling again at a lower room temperature.
	Inadequate air supply to ventilation unit.	Check distance of instrument to wall; it must be at least 15 cm.
	Refrigeration system leak.	The instrument must be serviced or repaired by a service technician. Please contact customer service.
Compressor switches off after a brief period of operation and switches back on shortly thereafter.	AC power does not match specifications or compressor defective.	Check the voltage of power supply.
Compressor does not start.	Compressor defective.	The instrument must be serviced or repaired by a service technician.
Compressor switches off after a short period of operation.	Compressor defective.	Please contact customer service.

6.1 Cleaning the instrument

Switch off the instrument each time before cleaning and pull out the mains plug.

While handling cleaning materials, observe the safety regulations of the manufacturer and the lab regulations valid in the country of use.

During cleaning, do not allow any liquid to penetrate inside the instrument!

To prevent scratching the surface of the instrument, do not use metallic tools with sharp edges under any circumstances.

Work surface

- All common laboratory cleaning products suitable for the removal of paraffin (e.g. Paraguard or xylene substitutes) can be used to clean the work area.

Instrument and exterior surfaces

• If necessary, clean the painted exterior surfaces with a mild household cleaner or soapy water and wipe with a damp cloth.
• Avoid prolonged contact of organic solvents on the surface of the instrument. Do not use xylol or acetone on the painted surfaces!

6.2 Maintenance instructions

Only Leica service technicians are authorized to open the instrument for maintenance and repair work.

Please observe the following points to ensure the instrument's reliable function over extended periods:

• Clean the instrument with care daily.
• Regularly remove dust from the ventilation slots on the back of the instrument with a brush or vacuum cleaner.
• Have the instrument inspected at least once a year by an authorized Leica customer service technician.
• Enter into a service contract at the end of the warranty period. For more information, contact the relevant Leica customer service organization.

Warranty

Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control procedure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/or agreed characteristics warranted.

The scope of the warranty is based on the content of the concluded agreement. The warranty terms of your Leica sales organization or the organization from which you have purchased the contractual product shall apply exclusively.

Service information

If you require technical service or replacement parts, please contact your Leica sales representative or dealer who sold the product.

Please provide the following information:

• Model name and serial number of the instrument.
• Location of the instrument and name of the person to contact.
• Reason for the service call.
• Date of delivery.

Decommissioning and disposal

The instrument or parts of the instrument must be disposed of in compliance with the local laws.