Levenhuk

MED PRO 600 Fluo - Microscope Levenhuk - Free user manual and instructions

Find the device manual for free MED PRO 600 Fluo Levenhuk in PDF.

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Product Type Fluorescence Microscope
Brand Levenhuk
Model MED PRO 600 Fluo
Magnification Range 40x – 1000x
Eyepiece WF10x/20 mm
Objective Lenses Plan Achromatic 4x, 10x, 40x, 100x (oil)
Fluorescence Illuminator 100W Mercury Lamp
Fluorescence Filter Sets DAPI, FITC, TRITC
Condenser Abbe NA 1.25 with iris diaphragm
Stage Mechanical, double layer, 140x140 mm
Focusing Coaxial coarse and fine, 0.002 mm per division
Power Supply 100-240V, 50/60 Hz
Dimensions (W x D x H) 400 x 300 x 500 mm
Weight Approx. 12 kg
Maintenance Clean optics with lens paper, store in dry environment
Safety Use UV protection, avoid direct skin exposure to lamp
Spare Parts Available Replacement bulbs, filters, objectives
Warranty 2 years
Applications Clinical, research, biological fluorescence imaging

Frequently Asked Questions - MED PRO 600 Fluo Levenhuk

How do I set up the microscope for fluorescence observation?
First, turn on the mercury lamp and allow it to warm up for 10-15 minutes. Select the appropriate filter set (e.g., DAPI, FITC, or TRITC) using the slider. Place the specimen on the stage, adjust focus in brightfield mode, then switch to fluorescence mode by rotating the fluorescence cube turret. Use the shutter to control excitation light.
What filter sets are included with the MED PRO 600 Fluo?
The microscope comes with three standard filter sets: DAPI (excitation 350/50 nm, emission 460/50 nm), FITC (excitation 480/30 nm, emission 535/40 nm), and TRITC (excitation 545/30 nm, emission 620/60 nm). Additional filters can be purchased separately.
How do I replace the mercury lamp?
Ensure the lamp is cool and the power is off. Open the lamp housing cover. Wear gloves to avoid oil from skin damaging the bulb. Remove the old lamp by unscrewing it, then insert the new lamp without touching the glass. Align the lamp using the centering screws for optimal illumination. Refer to the manual for detailed steps.
Can I use LED illumination instead of the mercury lamp?
The MED PRO 600 Fluo is designed for a 100W mercury lamp. While aftermarket LED converters may exist, they are not officially supported. Using an alternative light source may affect fluorescence intensity and spectral output. Always consult Levenhuk support before modifying.
How should I clean the objectives?
Use lens paper or a soft microfiber cloth moistened with optical cleaning solution (e.g., isopropyl alcohol 70%). Blow off dust first with a blower brush. Gently wipe in a circular motion from center outward. Avoid excessive pressure to prevent scratches.
What is the warranty period for this microscope?
Levenhuk offers a 2-year warranty against manufacturing defects for the MED PRO 600 Fluo. The warranty covers repairs or replacement of defective parts, but does not cover damage from misuse, accidents, or normal wear (e.g., lamp replacement).
Can I use oil immersion without immersion oil?
No, the 100x objective is designed for oil immersion only. Using it without oil will result in poor resolution and possible damage. Apply a drop of immersion oil (refractive index 1.515) to the cover slip before lowering the objective. Clean the objective immediately after use.
How do I adjust Koehler illumination?
First, focus on a specimen. Close the field diaphragm and adjust the condenser height until the diaphragm’s image is sharp. Center the image using the condenser centering screws. Open the field diaphragm until it just disappears from view. Then, adjust the aperture diaphragm to match the objective’s numerical aperture.
Is the MED PRO 600 Fluo suitable for live cell imaging?
Yes, with precautions. Use low-intensity excitation and short exposure times to minimize phototoxicity. A heated stage or CO2 incubation system may be needed to maintain cell viability. The microscope’s fluorescence capability allows imaging of labeled live cells.
How should I store the microscope when not in use?
Always cover the microscope with the dust cover provided. Store in a dry, cool environment away from direct sunlight. Ensure the objectives are clean and the focus knobs are loosened. Avoid storing near chemicals or in high humidity to prevent mold growth on optics.

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USER MANUAL MED PRO 600 Fluo Levenhuk

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Product information

Brand : Levenhuk

Model : MED PRO 600 Fluo

Category : Microscope